Use of Proteomic Analysis To Elucidate the Role of Calcium in Acetone-Butanol-Ethanol Fermentation by Clostridium beijerinckii NCIMB 8052

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  Total ABE concentration, yield, and productivity obtained during batch ABE fermentation by C. beijerinckii NCIMB 8052 (Table S1), isoelectric points (Table S2), full list of proteins which were significantly downregulated in Ca²⁺-supplemented C. beijerinckii 8052 ABE fermentation relative to the untreated cells (Table S3), representative expression levels of selected proteins of C. beijerinckii 8052, which increased or decreased in Ca²⁺-treated cells compared to the untreated cells (Fig. S1), and comparison of total ABE and butanol profiles between batch C. beijerinckii NCIMB 8052 fermentations conducted in P2 medium supplemented with 0.5 g/liter CaCl₂ and 0.5 g/liter CaCO₃ (Fig. S2).

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