Multiple Envelope Stress Response Pathways Are Activated in an Escherichia coli Strain with Mutations in Two Members of the DedA Membrane Protein Family

Supplemental material

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    Table S1, primers

    Supplemental materials and methods

    Fig. S1, repression of σE stress response in RS28AB by the activated Cpx pathway

    Fig. S2, addition of 10 mM Mg2+ and 400 mM NaCl or lowering of the growth medium pH partially attenuates Cpx activation in RS28AB while restoration of the PE level in RS28AB fails to attenuate Cpx activation

    Fig. S3, partial attenuation of Psp activation in RS28AB is observed when either 10 mM Mg2+ or 400 mM NaCl is added to the growth medium or when the cells are grown in rich medium at pH 6.0

    Fig. S4, attenuation of Rcs activation in RS28AB is observed in the presence of 10 mM Mg2+ or 400 mM NaCl or in growth medium with reduced pH

    Fig. S5, MdfA overexpression restores the growth of strains BC202C, BC202R, BC202F, and BC202bae at 43°C

    Fig. S6, partial attenuation of Bae activation in RS28AB is observed in the presence of 10 mM Mg2+ or 400 mM NaCl or in growth medium with reduced pH

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