Rapid Intracellular Competition between Hepatitis C Viral Genomes as a Result of Mitosis

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Suppl. Methods, Results, and References.

    Fig. S1 (Absolute cell counts of replicon-positive cells in dual-replicon decay experiments.)

    Fig. S2 (Decay of dual- and single-replicon cells in the presence and absence of triple-replicon cells.)

    Fig. S3 (Sorting procedures.)

    Fig. S4 and S5 (Persistence of single-replicon cells and decay of dual-replicon cells in Jc1/ΔXFP-BSD-transfected cells.)

    Fig. S6 (Jc1/ΔXFP-BSD single-replicon-positive cells that have an advantage in proliferation have a disadvantage in the decay process.)

    Table S1 (Primer sequences and templates used in plasmid construction.)

    Table S2 (Probe/primer sets used in quantitative real-time PCR.)

    Suppl. Video Legends

    PDF, 2.2M

  • Supplemental file 2 -

    Video S1 (Mitosis of a 7.5-EBFP2-hGem cell and attendant loss of EBFP2-hGem fluorescence after division.)

    AVI, 2.8M

  • Supplemental file 3 -

    Video S2 (Mitosis of a dual-replicon cell and a marked shift in fluorescence favoring the Jc1/ΔmCherry replicon in both of the daughter cells.)

    AVI, 11M

  • Supplemental file 4 -

    Video S3 (Mitosis of a dual-replicon cell and near complete loss of Jc1/ΔGFP fluorescence in both the daughter cells.)

    AVI, 8.2M

  • Supplemental file 5 -

    Video S4 (Mitosis of a dual-replicon cell and near complete loss of Jc1/ΔmKO2-BSD fluorescence in both of the daughter cells.)

    AVI, 11M

  • Supplemental file 6 -

    Video S5 (Initiation of mitosis in a dual-replicon cell with a failure to complete cytokinesis; a shift in fluorescence is observed favoring the Jc1/ΔmKO2-BSD replicon.)

    AVI, 6.6M

  • Supplemental file 7 -

    Video S6 (Mitosis of a dual-replicon cell and a marked shift in fluorescence favoring the Jc1/ΔmKO2-BSD replicon in only one of the daughter cells.)

    AVI, 11M