Video 1.
Largely synchronized fusion of primary myoblasts from WT mice. Primary myoblasts from WT mice were incubated for 8 h in DM and then for 16 h in DM supplemented with LPC. At t = 0 the cells were washed to remove LPC and lift the block to cell fusion and transferred into a microscope stage culture dish incubator for imaging. Images were analyzed by time-lapse phase-contrast microscopy using a custom setup as described in Materials and methods. Frames were taken every 2 min for 70 min. Time since removal of LPC is indicated as hours:minutes. Regions where myoblast fusion occurs are marked by yellow rectangles. Bar, 100 µm.