Video 2.
Control experiment that shows a few fusion events between WT primary myoblasts observed within the same time interval from 24 to 25 h in DM as in Video 1 for the cells that were not treated with LPC. Primary myoblasts from WT mice were incubated for 24 h in DM and then transferred into a microscope stage culture dish incubator for imaging. Images were analyzed by time-lapse phase-contrast microscopy using a custom setup described in Materials and methods. Frames were taken every 2 min for 66 min. Time since transfer onto microscope stage is indicated as hours:minutes. Regions where myoblast fusion occurs are marked by yellow rectangles. Bar, 100 µm.