Anaphase-promoting Complex/Cyclosome-mediated Proteolysis of Ams2 in the G₁ Phase Ensures the Coupling of Histone Gene Expression to DNA Replication in Fission Yeast

Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1 (.pdf, 85 KB) - Supplemental Table 1, Strains used in this study.
• Supplemental Figure S1 (.pdf, 570 KB) - Supplemental Figure S1. Ams2 is not a substrate of the APC/C-Cdc20.
• Supplemental Figure S2 (.pdf, 680 KB) - Supplemental Figure S2. Ams2-dKm activates histone transcription from G1.
• Supplemental Figure S3 (.pdf, 621 KB) - Supplemental Figure S3. Ams2 levels fluctuate during the cell cycle.
• Supplemental Figure S4 (.pdf, 4.1 MB) - Supplemental Figure S4. Sensitivity to DNA damaging agents of HDAC mutants and double mutants with ams2-dKm.
• Supplemental Figure S5 (.pdf, 3.5 MB) - Supplemental Figure S5. Effects of stability of Ams2 on genotoxic stresses.
• Supplemental Figure S6 (.pdf, 5.5 MB) - Supplemental Figure S6. Meiotic S phase delay in ams2-dKm is neither dependent on Cds1 nor Chk1.
• Supplemental Figure S7 (.pdf, 1.1 MB) - Supplemental Figure S7. A feedback mechanism to monitor Ams2 levels in G1.
• Supplemental Figure S8 (.pdf, 403 KB) - Supplemental Figure S8. HU activates transcription of ams2 and cdc22 regardless of the presence or absence of chk1+.