Systems Engineering of Tyrosine 195, Tyrosine 260, and Glutamine 265 in Cyclodextrin Glycosyltransferase from Paenibacillus macerans To Enhance Maltodextrin Specificity for 2-O-d-Glucopyranosyl-l-Ascorbic Acid Synthesis

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Lineweaver-Burk plots of AA-2G synthesis by the wild-type CGTase and the mutant Y260R (Fig. S1), close-up of the wild-type and mutant CGTase theoretical structures with superpositioning of maltononaose structure (Fig. S2), and schematic representation of the interactions between the mutant Y260R/Q265K/Y195S CGTase and wild-type sugar residues (Fig. S3).

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