Video 8.
Half-ring and/or full-ring bleaching of Mlc1-GFP and Iqg1-GFP in wild-type and myo1Δ cells during cytokinesis. Related to Fig. 7. (first and second columns, counting from the left) Strains: YEF6065 (CDC3-RFP, pUG34-MLC1; first column) and YEF6351 (myo1Δ CDC3-RFP, pUG34-MLC1; second column). Note that YEF6065 carries an ade2 mutation, which presumably accounts for the red appearance of the vacuoles. A 10-s time-lapse interval is shown. Green, Mlc1-GFP; red, Cdc3-RFP. (third and fourth columns) Strains: YEF6140 (CDC3-RFP, pUG35-IQG1; third column) and YEF6356 (myo1Δ CDC3-RFP, pUG35-IQG1 fourth column). 10- and 3-s time-lapse intervals are shown for YEF6140 and YEF6356, respectively. Green, Iqg1-GFP; red, Cdc3-RFP. WT, wild type.