Video 1.
Myosin VI SI restricts the movement of SGs near the plasma membrane. Myosin VI stable knockdown PC12 cells cotransfected with GFP-MyoVI-SIfull and NPY-mCherry were visualized after nicotine stimulation using time-lapse live-cell imaging using a TIRF microscope (Marianas and Everest; Intelligent Imaging Innovations, Inc.). The trajectory of a single NPY-mCherry–positive SG becomes restricted as its colocalization with GFP-MyoVI-SIfull increases. The video was compiled at two frames per second.