Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis -- Data Supplement - HTML Page - 05984Fig5Legend.htslp -- Proceedings of the National Academy of Sciences

Fig. 5.
Photo-induced movement in the wild type and each mutant under blue light irradiation. (A) Second-positive hypocotyl phototropism in etiolated seedlings. Hypocotyl curvatures of etiolated seedlings of wild types (WS and Ler), the quadruple mutant, and the +phot1, +phot1T, +phot2, and +phot2T mutants were measured after a 12-h exposure to unilateral blue light at the indicated fluence rates. Data and error bars represent the means ± SD from the average values of three independent experiments. (B) Stomatal opening under blue light irradiation. The stomatal aperture under blue light irradiation in the wild types (WS and Ler), the quadruple mutant, and the +cry1, +cry1T, +cry2, +cry2T, +phot1, +phot1T, +phot2, and +phot2T mutants was measured. Data and error bars represent the means ± SD from the average values of three independent experiments. (C) Chloroplast relocation under blue light irradiation. Chloroplast movement was observed under red light. Mesophyll cells were irradiated with a microbeam of low-intensity blue light for 20-60 min and with high-intensity blue light for 60-90 min (without blue light irradiation from 0 to 20 min). White circles in the cell represent the irradiated area.