The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator -- Data Supplement - HTML Page - 08676Fig6Legend.htslp -- Proceedings of the National Academy of Sciences

Supporting Figure 6 Fig. 6.
Identification of the VP16 binding domain in ARC92 (ARC92VBD). Fragments of human ARC92 were translated in vitro to generate ³⁵S-labeled protein and incubated with GST-VP16 beads. After extensive washing, specifically bound proteins were resolved by SDS/PAGE and visualized by autoradiography. The VP16 binding domain resides in a region between amino acids 402 and 590 of ARC92. (Upper) Proteins bound to GST-VP16. (Lower) 10% input of ARC92 fragments.