The Vibrio cholerae chitin utilization program -- Data Supplement - HTML Page - 08707Table5.htslp -- Proceedings of the National Academy of Sciences

Table 5. Quantitative real-time RT-PCR analysis of gene expression

Gene		Relative gene expression
		GlcNAc – 60m *		(GlcNAc)₂ – 60m *		Crab attached ^†		(GlcN)₂ – 60m *
		Array ^‡	Real-timePCR ^§	Array ^‡	Real-time PCR ^§	Array ^‡	Real-time PCR ^§	Array ^‡	Real-time PCR ^§
VC0409	MSHA pilin protein MshA (mshA)	1.0	0.85	1.1	0.85	1.2	0.88	1.2	0.86
VC0409	MSHA pilin protein MshA (mshA)		(0.80-0.90)		(0.76-0.95)		(0.79-0.93)		(0.73-1.01)
VC0449	Methyl-accepting chemotaxis protein	2.8	10.1	4.1	12.0	0.6	0.36	1.0	1.84
VC0449	Methyl-accepting chemotaxis protein		(9.07-11.3)		(10.8-13.5)		(0.30-0.43)		(1.53-2.21)
VC0620	Peptide ABC transporter, periplasmic peptide-binding protein	1.3	0.91	71	129	6.6	27.2	1.2	1.19
VC0620			(0.82-1.01)		(82.7-200)		(23.9-30.8)		(0.99-1.44)
VC0828	Toxin co-regulated pilin (tcpA)	1.1	1.57	1.3	1.65	0.8	0.98	1.0	1.02
VC0828	Toxin co-regulated pilin (tcpA)		(1.48-1.66)		(1.52-1.79)		(0.86-1.19)		(0.85-1.21)
VC0972	Chitoporin ChiP	2.4	2.82	30	202	8.4	31.1	1.2	1.50
VC0972	Chitoporin ChiP		(2.19-3.63)		(186-221)		(27.9-34.7)		(1.24-1.80)
VC1153	Conserved hypothetical protein	1.1	1.39	7.8	9.30	4.1	3.76	1.3	1.53
VC1153	Conserved hypothetical protein		(1.26-1.53)		(8.43-10.3)		(3.29-4.29)		(1.21-1.94)
VC1282	PTS system, cellobiose-specific IIC component (celB)	0.8	0.15	0.6	0.32	3.9	2.34	31	118
VC1282	PTS system, cellobiose-specific IIC component (celB)		(0.11-0.21)		(0.25-0.40)		(2.10-2.61)		(98-141)
VC2423	Fimbrial protein (pilA)	1.0	0.80	12	54.3	9.0	17.1	1.0	0.98
VC2423	Fimbrial protein (pilA)		(0.63-0.85)		(50.5-58.4)		(15.4-19.1)		(0.83-1.16)
VCA0027	Chitinase (chiA-2)	0.9	1.37	5.0	8.69	14	14.4	0.9	0.93
VCA0027	Chitinase (chiA-2)		(1.19-1.58)		(3.97-10.3)		(12.7-16.4)		(0.78-1.11)
VCA0140	Spindolin-related protein	0.9	0.72	2.6	4.28	7.5	3.37	0.9	1.06
VCA0140	Spindolin-related protein		(0.70-0.74)		(3.93-4.66)		(2.94-3.86)		(0.88-1.27)

*Fold induction relative to culture grown without the indicated inducer.

^†Fold induction of the attached population relative to the planktonic population. ^‡Average induction values from two arrays with same RNA sample. ^§Average induction values (and range of induction values) obtained from three replicas using same RNA sample as in ‡.