The GTPase Activity of FlhF Is Dispensable for Flagellar Localization, but Not Motility, in Pseudomonas aeruginosa

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Table S1, MS/MS data

    Fig. S1, SDS-PAGE analysis of refolded GST-FlhF-His6 protein preparations

    Fig. S2, Western blot to confirm expression of FlhF fusion proteins in bacterial two-hybrid assay

    Fig. S3, FlhF expression in complemented ΔflhF strains

    Fig. S4, all FlhF mutants restore polar flagellar assembly

    Fig. S7, DRaCALA assay of [α-32P]GTP binding over time

    PDF, 6.0M

  • Supplemental file 2 -

    Movie S5, PAK ΔflhF complemented with FlhF(R251G) on anti-FliC antibody-coated slides

    AVI, 9.2M

  • Supplemental file 3 -

    Movie S6, PAK ΔflhF complemented with FlhF(R251G) on BSA-coated slides

    AVI, 8.0M