Video 1.
Dynamics of GFP-EB1 in a control depleted RPE1 cell. RPE1 cells stably expressing GFP-EB1 were treated with control siRNA, serum starved for 24 h, and analyzed by time-lapse microscopy using a spinning-disk confocal microscope (UltraVIEW ERS; PerkinElmer). Time-lapse images were acquired at 400-ms intervals for 1 min at 37°C. A single control depleted RPE1 cell is shown. The video is played back at 12 frames/s.