LOCUS pDREV-2 6325 bp DNA circular 22-JUL-2010 SOURCE ORGANISM COMMENT ORIGDB|GenBank COMMENT VNTDATE|560443088| COMMENT VNTDBDATE|560452162| COMMENT LSOWNER| COMMENT VNTNAME|pDREV-2| COMMENT VNTAUTHORNAME|JLR| COMMENT VNTOAUTHORNAME|Javier Lopez-Rios| FEATURES Location/Qualifiers exon 1393..1426 /vntifkey="61" /label=En2_Exon2 /note="EN2_exon2" misc_recomb 359..457 /vntifkey="86" /label=Gateway\L1 /note="Gateway L1" misc_feature 518..1392 /vntifkey="21" /label=En2\intron /note="En2 intron" misc_feature 4980..5552 /vntifkey="21" /label=ClonNAT /note="ClonNAT" misc_feature 1442..1494 /vntifkey="21" /label=T2A misc_feature 2610..2860 /vntifkey="21" /label=SV40\pA misc_recomb complement(4661..4694) /vntifkey="86" /label=loxP misc_recomb 4717..4815 /vntifkey="86" /label=Gateway\L2 misc_recomb complement(2869..2900) /vntifkey="86" /label=Rox misc_recomb complement(4621..4652) /vntifkey="86" /label=Rox CDS 1502..1876 /vntifkey="4" /label=H2B\ORF CDS 1883..2602 /vntifkey="4" /label=Venus\ORF misc_feature 4910..4979 /vntifkey="21" /label=EM7\promoter CDS 3481..4080 /vntifkey="4" /label=puromycin\N-acetyl\transferase misc_feature 2922..4620 /vntifkey="21" /label=PGK-Puro-pA misc_feature 1427..1441 /vntifkey="21" /label=reading\frame\2 misc_recomb 484..517 /vntifkey="86" /label=FRT BASE COUNT 1441 a 1823 c 1701 g 1359 t 1 others ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaaccg aagttcctat 481 tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca caccaccctc 541 cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact aacagaagaa 601 cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg tcagttgggt 661 ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg gacaggccac 721 caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg acaggaagac 781 tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag tccttcaagg 841 aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc tactgccctt 901 gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc ttgactggct 961 agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga ccctttccct 1021 aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc agaaagattt 1081 tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga taggcctttt 1141 agctacatct gccaatccat ctcattttca cacacacaca caccactttc cttctggtca 1201 gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac acttgtatgg 1261 ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc tagcttgggt 1321 gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc ctttctcctc 1381 catgacaacc aggtcccagg tcccgaaaac caaagaagaa gaacgcagat cgcagatctc 1441 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gaccgggggg 1501 accagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa 1561 ggcgcagaag aaagacggca agaagcgcaa gcgcagccgc aaggagagct attccatcta 1621 tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca aggccatggg 1681 catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg cttcccgcct 1741 ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg ccgtgcgcct 1801 gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg ccgtcaccaa 1861 gtacaccagc gctaaatcca ccatggtgag caagggcgag gagctgttca ccggggtggt 1921 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 1981 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa 2041 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc 2101 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 2161 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 2221 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 2281 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 2341 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga 2401 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 2461 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 2521 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 2581 catggacgag ctgtacaagt aagaagagct taattaatga tcataatcag ccatatcaca 2641 tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 2701 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 2761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 2821 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtttaaacta actttaaata 2881 attggcatta tttaaagtta catatggcgg ccgccctaag gctcgacctc gaaattctac 2941 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg 3001 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg 3061 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 3121 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 3181 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 3241 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 3301 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtct 3361 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 3421 tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct gaagcttacc 3481 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 3541 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccagat 3601 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 3661 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 3721 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 3781 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 3841 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 3901 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 3961 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 4021 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 4081 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg 4141 atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga tgtccactaa 4201 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt 4261 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcntgctc 4321 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt 4381 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct 4441 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact 4501 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca 4561 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gctggtcgag 4621 taactttaaa taattggcat tatttaaagt taggcgcgcc ataacttcgt atagcataca 4681 ttatacgaag ttatgtcgag atatttaaat attagaccca gctttcttgt acaaagttgg 4741 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 4801 taaaatcatt atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt 4861 acattgcaca agataaaagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca 4921 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca 4981 tgggtaccac tcttgacgac acggcttacc ggtaccgcac cagtgtcccg ggggacgccg 5041 aggccatcga ggcactggat gggtccttca ctaccgacac cgtcttccgc gtcaccgcca 5101 ccggggacgg cttcaccctg cgggaggtgc cggtggaccc gcccctgacc aaggtgttcc 5161 ccgacgacga atcggacgac gaatcggacg acggggagga cggtgacccg gactctcgga 5221 cgttcgtcgc gtacggggac gacggcgacc tggcgggctt cgtggtcgtc tcgtactccg 5281 gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac cgggggcacg 5341 gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc ccgcgagcgg ggtgccgggc 5401 acctctggct ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac cggcggatgg 5461 ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg caccgcctcg gacggcgagc 5521 aggcgctcta catgagcatg ccctgcccct aagcacttcg tggccgagga gcaggactga 5581 cacgttcaga attggttaat tggttgtaac attattcaga ttgggccccg ttccactgag 5641 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5701 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5761 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5821 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5881 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 5941 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6001 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6061 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6121 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6181 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6241 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 6301 tttgctggcc ttttgctcac atgtt //