Gene Target ,Symbol,Left primer,Right primers, Strict Melt QC Pass
Alpl,Alpl ,cggatcctgaccaaaaacc,tcatgatgtccgtggtcaat,NO
Alx4 ,Alx4 ,aaagagggagcgctttgg,tccaggatgggttctgaatct,YES
Bglap,Bglap2 (osteocalcin),agactccggcgctacctt,ctcgtcacaagcagggttaag,YES
Bglap1 (Bglap),Bglap1 (Bglap),agactccggcgctacctt,caagcagggttaagctcaca,NO
BGN,Bgn ,caacctagtccaccaactaagca,caggggacacatggtcaat,YES
BMP2,Bmp2 ,cggactgcggtctcctaa,ggggaagcagcaacactaga,YES
BMP6,Bmp6 ,actgactagcgcgcagga,tgtggggagaactccttgtc,YES
BMP7,Bmp7,cgagaccttccagatcacagt,cagcaagaagaggtccgact,YES
Bmpr1a ,Bmpr1a ,tggctgtctgtatagttgctatgat,tgcttgagatactcttacaataatgct,NO
c-fos,Fos (c-fos),gggacagcctttcctactacc,gatctgcgcaaaagtcctgt,NO
Ccr6 ,Ccr6 ,ttcgccactctaatcagtaggac,tctggtgtagaaagggaagtgg,YES
Ccrn4l,Ccrn4l ,ttgtggacctgaggacagatt,ttgccttctccgagagctt,YES
CD105,Eng (CD105),cattgcacttggcctacga,gatgttgactcttggctgtcc,YES
CD11b,Itgam (Cd11b),caatagccagcctcagtgc,gagcccaggggagaagtg,NO
CD14,Cd14 ,aaagaaactgaagcctttctcg,agcaacaagccaagcacac,NO
CD166,Alcam (CD166),cttcagtgtttggggaatgg,ttatgccttcaggctgtcct,NO
CD19,Cd19 ,aaggtcattgcaaggtcagc,ctgggactatccatccacca,NO
CD29,Itgb1 (CD29),tggcaacaatgaagctatcg,atgtcgggaccagtaggaca,NO
CD31,Pecam1 (Cd31),cggtgttcagcgagatcc,cgacaggatggaaatcacaa,YES
CD44,Cd44 ,ctccttctttatccggagcac,tggctttttgagtgcacagt,YES
CD45,Ptprc (Cd45),aatggctcttcagagaccacata,agtcaggctgtggggaca,YES
Cd5 ,Cd5 ,cagtgcttccagaaaacaacg,accttgggctggaaatcag,YES
Cd6 ,Cd6 ,gatcgctggattgctgaca,gatggtgccatttttgtgct,NO
CD73,Nt5e (CD73),atgaacatcctgggctacga,gtccttccacaccgttatcaa,YES
CD79a,Cd79a ,gatgccagggggtctagaag,gttcaccgtcagggatgg,YES
Cd79b ,Cd79b ,tggtgctgtcttccatgc,ttgctggtaccggctcac,YES
CD90,Thy1 (CD90),gaaaactgcgggcttcag,ccaagagttccgacttggat,YES
Ckip1,Plekho1 (Ckip1),cgagacagagcaaaaatccaa,ccatctgaggtagaggtcgaa,YES
Col1a1,Col1a1 ,catgttcagctttgtggacct,gcagctgacttcagggatgt,NO
Col1a2,Col1a2 ,gcaggttcacctactctgtcct,cttgccccattcatttgtct,YES
Col3a1 ,Col3a1 ,tcccctggaatctgtgaatc,tgagtcgaattggggagaat,YES
Col5a1 ,Col5a1 ,ctacatccgtgccctggt,ccagcaccgtcttctggtag,YES
Col5a2 ,Col5a2 ,gaaaggctggtgatcaaggt,tttctccccgaggtcctaat,NO
Col6a1 ,Col6a1 ,gacatccagggctccaaa,aggtgtcgagcacgaagaat,YES
Ctnnb1,Ctnnb1 ,gcagcagcagtttgtgga,tgtggagagctccagtacacc,YES
CXCL12,Cxcl12 ,ctgtgcccttcagattgttg,taatttcgggtcaatgcaca,YES
CXCR4,Cxcr4 ,tggaaccgatcagtgtgagt,gggcaggaagatcctattga,YES
Dlx5 ,Dlx5 ,agcccctaccaccagtacg,gctccgccacttctttctc,YES
Dmp1 ,Dmp1 ,ggttttgaccttgtgggaaa,ttgggatgcgattcctctac,NO
EPO,Epo ,tctgcgacagtcgagttctg,cttctgcacaacccatcgt,YES
Fmod ,Fmod ,cagggcaacaggatcaatg,ctgcagcttggagaagttcat,NO
Foxo1,Foxo1 ,cttcaaggataagggcgaca,gacagattgtggcgaattga,YES
Foxo3,Foxo3 ,gctaagcaggcctcatctca,ttccgtcagtttgagggtct,YES
Foxo4,Foxo4 ,aaggacaagggtgacagcaa,ctgtgcaaggacaggttgtg,YES
Glut4,Slc2a4,gacggacactccatctgttg,gccacgatggagacatagc,NO
HLA-DR,H2-Ea-ps,tcactggggtttggagga,gagcacacacgacattctctg,YES
IBSP,Ibsp ,gaaaatggagacggcgatag,cattgttttcctcttcgtttga,YES
Igf1,Igf1 ,caaaagcagcccgctcta,tcgatagggacggggact,YES
Igfbp2,Igfbp1 ,ttcagctcccagcatgaag,gggctctcagaaagctcatc,YES
IGFr1,Igf1r ,gagaatttccttcacaattccatc,cacttgcatgacgtctctcc,YES
leptin receptor,Lepr,gatgttccaaaccccaagaa,ttctgcatgcttggtaaaaaga,YES
Lhx2 (ap),Lhx2 (ap),cagcttgcgcaaaagacc,taaaaggttgcgcctgaact,YES
Lum ,Lum ,cgctcctcagctcttgtatca,catatgctgtgaactctgtcagg,YES
mCSF,Csf1 (MCSF),caacagctttgctaagtgctcta,cactgctaggggtggcttta,NO
Mgp ,Mgp ,gtcctatgaaatcagtcccttca,ttgttgcgttcctggactct,YES
MMP13,Mmp13 ,gccagaacttcccaaccat,tcagagcccagaattttctcc,YES
Mmp14 ,Mmp14 ,aacttcgtgttgcctgatga,tttgtgggtgaccctgactt,YES
Msx1 ,Msx1 ,cagagtccccgcttctcc,gtcttgtgcttgcgtaggg,YES
MSX2,Msx2 ,aggagcccggcagatact,gtttcctcagggtgcaggt,YES
notch1,Notch1 ,ctggaccccatggacatc,aggatgactgcacacattgc,NO
notch3,Notch3 ,agctgggtcctgaggtgat,agacagagccggttgtcaat,YES
notch4,Notch4 ,ggacctgcttgcaaccttc,cctcacagagcctcccttc,YES
Osteopontin,Spp1 (osteopontin),ggaggaaaccagccaagg,tgccagaatcagtcactttcac,YES
Osterix (sp7),Sp7 ,ctcctgcaggcagtcctc,gggaagggtgggtagtcatt,YES
p53,Trp53 (p53),atgcccatgctacagaggag,agactggcccttcttggtct,YES
Pgc1a,Ppargc1a,gaaagggccaaacagagaga,gtaaatcacacggcgctctt,YES
pleiotropin receptor,PTPRZ1,ggcactcaggagtatccaaca,gaccaatacgagactcatggcta,YES
Postn,Postn ,cgggaagaacgaatcattaca,accttggagacctctttttgc,YES
PPARG,Pparg ,gaaagacaacggacaaatcacc,gggggtgatatgtttgaacttg,YES
RANK,Tnfrsf11a (Rank),agaggcattatgagcatctcg,ggagtgcacttagaggacaggt,YES
RANKL,Tnfsf11 (RANKL),tgaagacacactacctgactcctg,ccacaatgtgttgcagttcc,YES
Runx2,Runx2 ,gcccaggcgtatttcaga,tgcctggctcttcttactgag,YES
slug,Snai2 (Slug),tgcaagatctgtggcaagg,cagtgagggcaagagaaagg,NO
SMAD1,Smad1 ,aacaccaggcgacatattgg,cactgaggcattccgcata,YES
SMAD5,Smad5 ,ccgagctgctaataaagttgc,caggtgccataggcttcttc,YES
Smurf1 ,Smurf1 ,aggggaggaaggtttggac,tcatggcacaacaaatacagc,YES
Snail1,Snai1 (Snail1),gtctgcacgacctgtggaa,caggagaatggcttctcacc,NO
Sox9,Sox9 ,cagcaagactctgggcaag,tccacgaagggtctcttctc,NO
SP1,Sp1 ,atgcccctattgcaaagaca,tggatgtgacaaatgtgctg,YES
SPARC,Sparc ,gcccctcagcagactgaa,ggttggcacccacaggta,NO
Sqstm1 ,Sqstm1 ,gaagctgccctatacccaca,tgggagagggactcaatcag,YES
Tbx3,Tbx3 ,ttgcaaagggttttcgagac,tgcagtgtgagctgctttct,YES
VCAN,Vcan ,tcctgattggcattagtgaaga,tttgttttgcagagatcaggtc,YES
VDR,Vdr ,cacctggctgatcttgtcagt,ctggtcatcagaggtgaggtc,NO
wif1,Wif1 ,ggcagacactgcaataagagg,ttaagtgaaggcgtgtgtcg,YES
Wisp1 ,Wisp1 ,tggacatccaactacacatcaag,aagttcgtggcctcctctg,YES
wnt1,Wnt1 ,acagtagtggccgatggtg,cttggaatccgtcaacaggt,YES
Wnt10b,Wnt10b ,gtggatcctgcacctgaac,cctccagcatgtcgaagc,YES
wnt5a,Wnt5a ,acgcttcgcttgaattcct,cccgggcttaatattccaa,YES