Single Amino Acid Substitutions in HXT2.4 from Scheffersomyces stipitis Lead to Improved Cellobiose Fermentation by Engineered Saccharomyces cerevisiae

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  Synthetic oligonucleotides used for sequencing of the evolved pRS426-HXT2.4 plasmid (Table S1), comparison of cellobiose fermentation profiles by CEN-HXT2.4-BGL and CEN-CDT1-BGL (Fig. S1), specific growth rates by CEN-HXT2.4-BGL (Fig. S2), predicted 3-D structure of of HXT2.4 (Fig. S3), cellobiose fermentation profiles by by CEN-HXT2.4-BGL, CEN-evolved_HXT2.4-BGL, and CEN-mutant_HXT2.4 (A291D)-BGL (Fig. S4), cellobiose fermentation profiles by ten mutants (Fig. S5), cellobiose fermentation profiles by nineteen transformants (Fig. S6), comparison of cellobiose consumption rates, ethanol production rates, and maximum accumulated cellodextrin concentrations by transformants (Fig. S7), and confirmation of membrane localizations (Fig. S8).

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