CLUSTAL W (1.83) multiple sequence alignment Hxk1p, Hexokinase A Pgi1p, Phosphoglucose Isomerase Pgm2p, Phosphoglucomutase Fbp1p, Fructose-1,6-Bisphosphatase Icl1p, Isocitrate Lyase Mls1p, Malate Synthase Ach1p, Acetyl CoA Hydrolase Atp2p, Mitochondrial ATP Synthase Subunit Beta Om45p, Mitochondrial Outer Membrane Protein Hsp12p, Heat Shock Protein Hsp26p, Heat Shock Protein Hsp30p, Heat Shock Protein Hxk1p ------------------------------------------------------------ Pgi1p ------------------------------------------------------------ Pgm2p ------------------------------------------------------------ Fbp1p ------------------------------------------------------------ Icl1p ------------------------------------------------------------ Mls1p MVKVSLDNVKLLVDVDKEPFFKPSSTTVGDILTKDALEFIVLLHRTFNNKRKQLLENRQV Ach1p ------------------------------------------------------------ Atp2p MV---------------------------------------------------------- Om45p ---------------------------------------------MSSRIIVGSAALAAA Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p ------------------------------------------------------------ Pgi1p ------------------------------------------------------------ Pgm2p ------------------------------------------------------------ Fbp1p ------------------------------------------------------------ Icl1p ------------------------------------------------------------ Mls1p VQKKLDSGSYHLDFLPETANIRNDPTWQGPILAPGLINRSTEITGPPLRNMLINALNAPV Ach1p ------------------------------------------------------------ Atp2p ------------------------------------------------------------ Om45p ITASIMVREQKAKGQRREGNVSAYYNGQEYGSSAPPQLGKLHNIKQGIKEDALSLKDALL Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p ------------------------------------------------------------ Pgi1p ------------------------------------------------------------ Pgm2p ------------------------------------------------------------ Fbp1p ------------------------------------------------------------ Icl1p ------------------------------------------------------------ Mls1p NTYMTDFEDSASPTWNNMVYGQVNLYDAIRNQIDFDTPRKSYKLNGNVANLPTIIVRPRG Ach1p ------------------------------------------------------------ Atp2p ------------------------------------------------------------ Om45p GVSQKAREEAPKVTKRVISPEEDAQTRKQLGQKAKDSSSQSIFNWGFSEAERRKAIAIGE Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p ----------------------------------------------------------MV Pgi1p ------------------------------------------------------MSNNSF Pgm2p ------------------------------------------------------MSF-QI Fbp1p ------------------------------------------------------------ Icl1p ------------------------------------------------------------ Mls1p WHMVEKHLYVDDEPISASIFDFGLYFYHNAKELIKLGKGPYFYLPKMEHHLEAKLWNDVF Ach1p ------------------------------------------------------------ Atp2p ------------------------------------------------------------ Om45p FDTAKKRFEEAVDRNEKELLSTVMREKKAALDRASIEYERYGRARDF-NELSDKLDQQER Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p HLGPKKPQARKGS-MADVPKELMQQIENFEKIFTVPTETLQAVTKHFISELEKGLS---- Pgi1p TNFKLATELPAWS-KLQKIYESQGKTLSVKQEFQKDAKRFEKLNKTFTNYDGSKIL---- Pgm2p ETVPTKPY--------EDQKPGTSGLRKKTKVFKDEPNYTENFIQSIMEAIPEGSK---- Fbp1p -----------------MPTLVNGPRRDSTEGFDTDIITLPRFIIEHQKQFKNATG---- Icl1p --MPIPVGNTKND-FAALQAKLDADAAEIEKWWSDSRWSKTKRNYSARDIAVRRGT---- Mls1p CVAQDYIGIPRGTIRATVLIETLPAAFQMEEIIYQLRQHSSGLNCGRWDYIFSTIKRLRN Ach1p ------------------------------------------------------------ Atp2p --------LPR------LYTATSRAAFKAAK----------------------------- Om45p NSNPLKRLLKNNT---GDANTEEAAARSVQGWGDTAQE-FGREELEEAKRNASSEP---- Hsp12p -------------------------------MSDAGRKGFGEKASEALKPDSQKSY---- Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p -----KKGGNI-----PMIPGWVMDFPTGKESGDF-------LAIDLGGTNLRVVLVKLG Pgi1p -----FDYSKN-----LVNDEIIAALIELAKEANV-------TG--LRDAMFKGEHINST Pgm2p -----GATLVVGGDGRYYNDVILHKIAAIGAANGI-------KKLVIGQHGLLSTPAASH Fbp1p -----DFTLVL-----NALQFAFKFVSHTIRRAEL-------VN-LVGLAGAS----NFT Icl1p -------FPPIEYPSSVMARKLFKVLEKHHNEGTV-------SKTFGALDPVQISQMAKY Mls1p DPNHILPNRNQVTMTSPFMDAYVKRLINTCHRRGVHAMGGMAAQIPIKDDPAANEKAMTK Ach1p ------MTISNLLKQRVRYAPYLKKVKEAHELIPL-----FKNGQYLGWSGFTGVGTPKA Atp2p -------------QSAPLLSTSWKRCMASAAQS--------------------------- Om45p --------------SEAQKR--LDELKKIKEKGWF--------GYNKGE-QSEQQIAERV Hsp12p --------------AEQGKEYITDKADKVAGKVQP--------EDNKGVFQGVHDSAEKG Hsp26p ----------M-----SFNSPFFDFFDNINNEVDA-------FNRLLGEGGLR----GYA Hsp30p ------------------------------------------------------------ Hxk1p GDRTF------------DTTQSKYRL-PDAMRTTQNPDELWEFIADSLKAFID------- Pgi1p EDRAV------------YHVALRNRANKPMYVDGVNVAPEVDSVLKHMKEFSEQVRSGEW Pgm2p IMRTYEEKCTGGIILTASHNPGGPENDMGIKYNLSNGGPAPESVTNAIWEISKKLTS--Y Fbp1p GDQQK------------KLDVLGDEIFINAMRASGIIKVLVSEEQE-------------- Icl1p LDTIYISGWQCSSTASTSNEPGPDLADYPMDTVPNKVEHLFKAQLFHDRKQLEARSKA-- Mls1p VRNDKIRELTNGHDGSWVAHPALAPICNEVFINMGTPNQIYFIPENVVTAANLLETKI-- Ach1p VPEALIDHVEK------NNLQGKLRFNLFVGASAGPEENRWAEHDMIIKRAPHQVGKP-- Atp2p -----------------------TPITGKVTAVIGAIVDVHFEQSELPAILNALEIKT-- Om45p ARGLEGWGETAAQLSKDEMDDLRWNYENSKKQLDKNVSDAMDSLSKAKEDLKQYGSHW-W Hsp12p KDNAEGQGESLADQARDYMGAAKSKLNDAVEYVSGRVHGEEDPTKK-------------- Hsp26p PRRQL------------ANTPAKDSTGKEVARPNNYAGALYDPRDETLDDWFDN------ Hsp30p -------------------------------------------MNDTLSSFLNRNEAL-- Hxk1p -----------EQFPQGISE-P---IPLGFTFSFPASQNKINEGILQRWTKGFDIPNIEN Pgi1p KGYTGKKITDVVNIGIGGSD-L---GPVMVTEALKHYAGVLDVHFVSNIDGTHIAETLKV Pgm2p KIIKDFPELDLGTIGKNKKYGP---LLVDIIDITKDYVNFLKEIFDFDLIKKFIDNQRST Fbp1p ---------DLIVFPTNTG-------SYAVCCDPIDGSSNLDAGVSVGTIAS-------- Icl1p -----KSQEELDEMGAPIDYLT-----PIVADADAGHGGLTAVFKLTKMFIERGAAGIHM Mls1p ----PNGEITTEGIVQNLD------IGLQYMEAWLRGSG--------CVPIN-------- Ach1p -IAKAINQGRIEFFDKHLSMFP-QDLTYGFYTRERKDNKILDYTIIEATAIKEDGSIVPG Atp2p ----PQGKLVLE-VAQHLGENTVRTIAMDGTEGLVRGEKVLDTGGPISVPVGRETLGRII Om45p SGWTSKVDNDKQALKDEAQKKY-DEALKKYDEAKNKFKEWNDKGDGKFWSSKKD------ Hsp12p ------------------------------------------------------------ Hsp26p ---------DLSLFPSGFGF-P---RSVAVPVDILDHDNNYELKVVVPGVKSKKDIDIEY Hsp30p -GLNPPHGLDMHITKRGSDWLW-------------AVFAVFGFILLCYVVMFFIAENKGS Hxk1p HDVVPMLQKQITKRNIPIEVVALINDTTGTLVASYYTDPE-------------------- Pgi1p VDPETTLFLIASKTFTTAETITNANTAKNWFLSKTGNDPSHIAKH--FAALSTNETEVAK Pgm2p KNWKLLFDSMNGVTGPYGKAIFVDEFGLPADEVLQNWHPS---PD--FGGMHPDPN--LT Fbp1p -----IFRLLPDSSGTINDVLRCGKEMVAACYAMY--GSS-------------------- Icl1p EDQTSTNKKCGHMAGRCVIPVQEHVNRLVTIRMCADIMHSDLIVVA---RTDSEAATLIS Mls1p --------------------------NLMEDAATAEVSRC-------------------- Ach1p PSVGGSPEFITVSDKVIIEVNTATPSFEGIHDIDMPVNPPFRKPYP--YLKVDDKCGVDS Atp2p NVIGEPIDERGPIKSKLRKPIHADPPSFAEQSTSAEILETGIKVVDLLAPYARGGKIGLF Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p HQNKNQILVSGEIPSTLNEESKDKVKVKESSSGKF--KRV-------------------- Hsp30p RLTRYALAPAFLITFFEFFAFFTYASDLGWTGVQAEFNHVKVSKSI-------------T Hxk1p ------------------------TKMGVIFGTGVNGAYYDVCSDIEK----LQGKLSDD Pgi1p FGIDTKNMFGFESWVGGRYSVWSAIGLSVALYIGYDNFEAFLKGAEAVDNHFTQTPLEDN Pgm2p YASSLVKRVDREK-----------IEFGAASDGDGDRNMIYGYGPSFV------SPGDSV Fbp1p ------------------------THLVLTLGDGVDGFTLDTNLGEFI----LTHPNLRI Icl1p STIDTRDHYFIVGATNPNIEPF-AEVLNDAIMSGASGQELADIEQKWCRDA--GLKLFHE Mls1p -----------------------------------------QLYQWVKH----GVTLKDT Ach1p IPVDPEKVVAIVESTMRDQVPP--NTPSDDMSRAIAGHLVEFFRNEVKHGRLPENLLPLQ Atp2p GGAGVGKTVFIQELINNIAKAH--GGFSVFTGVGERTREGNDLYREMKE----TGVINLE Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p ------------------------ITLPDYPGVDADNIKADYANGVLT----LTVPKLK- Hsp30p GEVPGIRQIFYSKYIAWFLSWP-CLLFLIELAASTTGENDDISALDMVHSL----LIQIV Hxk1p IPPSAPMAINCEYGSFDNEHVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLA Pgi1p IPLLGGLLSVWYNNFFGAQTHLVA--PFDQYLHRFPAYLQQLSMESNGKSVTRGNVFTDY Pgm2p AIIAEYAAEIPYFAKQGIYGLARSF-PTSGAIDRVAKAHGLNCYEVPTGWKFFCALFDAK Fbp1p PPQKA----IYSINEGNTLYWNETIRTFIEKVKQPQADNNNKPFSARYVGSMVADVHRTF Icl1p AVIDEIERSALSNKQELIKKFTSKVGPLTETSHREAKKLAKEILGHEIFFDWELPRVREG Mls1p GEKVTPELTEKI------------------------------------------------ Ach1p SGIGNIANAVIEGLAGAQFKHLTVWTEVLQDSFLDLFENGSLDYATATSVRLTEKGFDRA Atp2p GESKVALVFGQMNEPPGARARVALTGLTIAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSA Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p -PQKDGKNHVKKIEVSSQESWGN------------------------------------- Hsp30p GTLFWVVSLLVGSLIKSTYKWGYYTIGAVAMLVTQGVICQRQFFNLKTRGFNALMLCTCM Hxk1p L------MDMYKQGFIFKNQDLSKFDKPFVM--DTSYPARI-------EEDPFENLEDTD Pgi1p STG---SILFGEPATNAQHSFFQLVHQGTKLIPSDFILAAQ-------SHNPIENKLHQK Pgm2p KL-----SICGEESFGTGSNHVREKDGVWAIMAWLNILAIY-------NKHHPENEASIK Fbp1p LYG---GLFAYPCDKKSPNGKLRLLYEAFPM--AFLMEQAG-------GKAVNDRGERIL Icl1p LYRYR---GGTQCSIMRARAFAPYADLVWMESNYPDFQQAKE-----FAEGVKEKFPDQW Mls1p ---------------------LKEQVERLSKAS--------------------------- Ach1p FANWE----NFKHRLCLRSQVVSNNPEMIRRLGVIAMNTPVEVDIYAHANSTNVNGSRML Atp2p LLGRIPSAVGYQPTLATDMGLLQERITTTKKGSVTSVQAVYVPADDLTDPAPATTFAHLD Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p VIVW---LYFICWGLSDGGNRIQPDGEAIFYGVLDLCVFAIYPCYLLIAVSRDGKLPRLS Hxk1p DLFQNEFGINTTV--Q----ERKLIRRLSELIGARAARLSVCGIAAICQKRGYKTGHIAA Pgi1p MLASNFFAQAEALMVG----KDEEQVKAEGATGGLVPHKVFSGNRPTTSILAQKITPATL Pgm2p TIQNEFWAKYGRTFFTRYDFEKVETEKANKIVDQLRAYVTKSGVVNSAFPADESLKVTDC Fbp1p DLVPSHIHDKSSIWLG----SSGEIDKFLDHIGKSQ------------------------ Icl1p LAYNLSPSFNWPKAMSVD--EQHTFIQRLGDLGYIWQFITLAGLHTNALAVHNFSRDFAK Mls1p -------------------PLGDKNKFALAA----------------------------- Ach1p NGLGGSADFLRNAKLSIM-HAPSARPTKVDPTGISTIVPMASHVDQTEHDLDILVTDQGL Atp2p ATTVLSRGISELGIYPAVDPLDSKSRLLDAAVVGQEHYDVASKVQETLQTYKSLQDIIAI Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p LTGGFSHHHATDDVEDAAPETKEAVPESPRASGETAIHEPEPEAEQAVEDTA-------- Hxk1p DGS-VYNRYPGFKEKAANALKDIYGWTQTSLDDYPIKIVPAEDGSGAGAAVIAALAQKR- Pgi1p GAL-IAYYEHVTFTEGAIWNINSFDQWGVELGKVLAKVIGKELDNSSTISTHDASTNGL- Pgm2p GDF-SYTDLDGSVSDHQGLYVKLSNGARFVLRLSGTGSSGATIRLYIEKYCDDKSQYQK- Fbp1p ------------------------------------------------------------ Icl1p DGM--KAYAQNVQQREMDDGVDVLKHQKWSGAEYIDGLLKLAQGGVSATAAMGTGVTED- Mls1p --------KYFLPEIRGEKFSEFLTTLLYDEIVSTKATPTDLSKL--------------- Ach1p ADLRGLSPKERAREIINKCAHPDYQALLTDYLDRAEHYAKKHNCLHEPHMLKNAFKFHT- Atp2p LGMDELSEQDKLTVERARKIQRFLSQPFAVAEVFT-GIPGKLVRLKDTVASFKAVLEGKY Om45p ------------------------------------------------------------ Hsp12p ------------------------------------------------------------ Hsp26p ------------------------------------------------------------ Hsp30p ------------------------------------------------------------ Hxk1p --IAEGKSVGIIGA-------------------- Pgi1p --INQFKEWM------------------------ Pgm2p --TAEEYLKPIINSVIKFLNFKQVLGTEEPTVRT Fbp1p ---------------------------------- Icl1p -QFKENGVKK------------------------ Mls1p ---------------------------------- Ach1p -NLAEKGTMKVDSWEPVD---------------- Atp2p DNIPEHAFYMVGGIEDVVAKAEKLAAEAN----- Om45p ---------------------------------- Hsp12p ---------------------------------- Hsp26p ---------------------------------- Hsp30p ----------------------------------