Fig. 5.

Combined inhibition of MET and FGFR leads to synergistic impairment of the growth of HNSCC cell lines. (A) UMSCC25 cells were treated in triplicate with DMSO, 0.1 μM PF1066, or 0.5 μM PF1066 alone or in combination with 0.5 μM AZ8010 or 1.0 μM AZ8010. The effect of the treatments on cell growth was measured by clonogenic assay. (B) UMSCC25 cells were plated in triplicate and treated with DMSO, AZ8010, PF1066, and the combinations at the doses shown. Proliferation was measured by CyQuant assay as described in the Materials and Methods and plotted on the top chart. Data are the means of two independent experiments. Combination indices (CIs) were calculated and shown on the bottom table. (C) As in (A), except 584-A2 cells were treated with DMSO, 0.5 μM PF1066, 30 nM AZ8010, and the combination and growth was measured by clonogenic assay. CCL30 cells were treated with DMSO, 0.3 μM PF1066, 0.1 μM AZ8010, and the combination and growth was measured by anchorage independent growth assay. (D) As in (A), except UMSCC1 and UMSCC8 cells were treated with DMSO, 0.3 μM PF1066, 0.3 μM AZ8010, and the combination and growth was measured by clonogenic assay. Data are the means and S.E.M. of three independent experiments. Statistical analysis using a two-tailed t test was used to show significant differences in growth. ns, not significant. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001.