Evidence for dynein and astral microtubule–mediated cortical release and transport of Gα_i/LGN/NuMA complex in mitotic cells

Supplemental Materials

This article contains the following supporting material:

• Supplemental Materials
• Movie01 - Supplementary movie 1: Time-lapse FRAP analysis of cortical Venus-LGN in an untreated cell.
• Movie02 - Supplementary movie 2: Time-lapse FRAP analysis of cortical Venus-LGN in a cell treated with 50 nM of nocodazole.
• Movie03 - Supplementary movie 3: Time-lapse FRAP analysis of cortical Venus-LGN in a cell transfected with DYNC1H1 shRNA. Supplementary movies correspond to Figure 4A.