Streptomyces lividans Blasticidin S Deaminase and Its Application in Engineering a Blasticidin S-Producing Strain for Ease of Genetic Manipulation

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  Bacterial strains, plasmids, and cosmids (Table S1); primers used in this research (Table S2); maps of the plasmids used in the construction of S. lividans LL2 (Fig. S1); bioassay of the bls gene cluster engrafted strain, S. lividans LL2 (Fig. S2); Q-TOF MS confirmation of deaminohydroxyblasticidin S (Fig. S3); protein alignment of SLBSD and its homologue in S. lividans TK24 (Fig. S4); confirmation of ΔSLBSD mutant S. lividans WJ1, and BS resistance of WJ1, WJ2, and HXY16 (Fig. S5); Q-TOF MS confirmation of demethylblasticidin S (Fig. S6); assay of blasticidin S production by the ΔblsF mutant strain S. lividans WJ4 (Fig. S7) and by the ΔblsE mutant strain S. lividans WJ5 (Fig. S8).

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