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Schematic representation of the genes responsible for the initial steps of nicotine degradation from Pseudomonas sp. strain HZN6. (A) (Left) Organization of the cloned genes. The short black line above the nox gene represents the region for RT-PCR analysis. (Right) Nicotine-degrading abilities of E. coli DH5α and P. putida KT2440 harboring different plasmids. +, ability; −, no ability. (B) Proposed initial steps of nicotine catabolism.
