Fig 4.

Agarose gel electrophoresis of RT-PCR products. The analysis was performed using total RNA isolated from strain HZN6, DH-nox, or KT-nox grown in MSM with nicotine (lane nic) or glucose (lane G). Control reactions: lane −, negative controls, DNase-treated RNAs from strain HZN6, DH-nox, or KT-nox grown in the presence of nicotine and used as templates; lane +, positive controls, genomic DNAs from strain HZN6, DH-nox, or KT-nox used as templates. The sizes of the amplified fragments were confirmed by comparison with the DL2000 marker (lane M).