Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie 1. Fluorescence recovery of EGFP-UAP56. HeLa cells transiently expressing EGFP-UAP56 were imaged by confocal laser scanning microscopy. EGFP-UAP56, concentrated at a RNA spicing nuclear speckled domain, was photobleached (white square). After bleaching, single images were acquired every 6 seconds for 115 seconds. This movie is from one of the cells analyzed in Figure 1
- Movie02 - Movie 2. Fluorescence recovery of EGFP-ALY/REF. HeLa cells transiently expressing EGFP-ALY/REF were imaged by confocal laser scanning microscopy. A region containing nucleoplasm and few RNA splicing speckled domains was photobleached (white square). After bleaching, single images were acquired every 6 seconds for 85 seconds. This movie is from one of the cells analyzed in Figure 1
- Movie03 - Movie 3. Fluorescence recovery of EGFP-NXF1. HeLa cells transiently expressing EGFP-NXF1 were imaged by confocal laser scanning microscopy. A region containing EGFP-NXF1 was bleached (white square) and the fluorescence recovery of that region was measured. After bleaching, single images were acquired every 6 seconds for 85 seconds. This movie is from one of the cells analyzed in Figure 1
- Movie04 - Movie 4. Fluorescence recovery of EGFP-eIF4A3. HeLa cells transiently expressing EGFP-eIF4A3 were imaged by confocal laser scanning microscopy. EGFP-eIF4A3, concentrated at a single RNA solicing speckled domain, was bleached (white square). After bleaching, single images were acquired every 6 seconds for 85 seconds. This movie is from one of the cells analyzed in Figure 2
- Movie05 - Movie 5. Fluorescence recovery of EGFP-MAGOH. HeLa cells transiently expressing EGFP-MAGOH were imaged by confocal laser scanning microscopy. The EGFP-MAGOH concentrated at a single RNA splicing speckled domain was bleached (white square). After bleaching, single images were acquired every 6 seconds for 85 seconds. This movie is from one of the cells analyzed in Figure 2.
- Movie06 - Movie 6. Fluorescence recovery of EGFP-Y14. HeLa cells transiently expressing EGFP-Y14 were imaged by confocal laser scanning microscopy. The EGFP-eIFY14 concentrated at a single RNA splicing speckled domain, was bleached (white square). After bleaching, single images were acquired every 6 seconds for 85 seconds. This movie is from one of the cells analyzed in Figure 2.