Phenotype Overlap in Xylella fastidiosa Is Controlled by the Cyclic Di-GMP Phosphodiesterase Eal in Response to Antibiotic Exposure and Diffusible Signal Factor-Mediated Cell-Cell Signaling

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  Primers used in this study (Table S1); analysis of kanamycin resistance gene [aph(3’)II] copy numbers in genomes of X. fastidiosa wild-type, rpfF mutant, and eal mutant strains by Southern blot hybridization (Fig. S1); reversion of the hypermotile phenotype of an eal mutant of X. fastidiosa by expression of eal from its native promoter in trans (Fig. S2); verification of X. fastidiosa Eal cyclic di-GMP phosphodiestrase activity (Fig. S3); effect of tobramycin on growth and biofilm formation of the wild type X. fastidiosa strain (Fig. S4).

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