The ana, hma, and tms mutants displayed a ratio of kanamycin-resistant to sensitive seedlings of » 1:2, 1:6 ,and 1:1, respectively. Normally segregating insertions that do not affect gametophytic transmission are expected to yield a 3:1 ratio, whereas a distorted 2:1 or 1:1 ratio is expected for insertions causing embryo- or sex-specific gametophytic lethality, respectively. To determine the transmission defects of the mutant alleles, reciprocal crosses of the heterozygous mutants with WT plants were performed and the percentage of kanamycin resistance in the progeny, indicating the frequency of transmission of the Ds element, was determined.