Fig. 6. (A) PCR amplification of the full-length Ds element from ana, hma, and tms. Primary or digested products were loaded. The EcoRI and BamH1 digest allowed confirmation of intact Ds elements (B) Schematic representation of the Ds insertion in each mutant. The primers used for PCR analysis and their position on genomic BAC clones flanking the Ds elements are indicated. Thick bidirectional arrows: Ds element and its annotation number in the starter line; short unidirectional arrows: primers; thin bidirectional arrows and fragment size: expected product sizes after EcoRI, BamHI digestion; Ds5' probe used for Southern analysis.