Fig. 7. Marker analysis of hma and tms Landsberg erecta (Ler)/Columbia (Col) F₁ hybrids. Physically anchored polymorphic markers were used to determine the extent of the deletion in hma (A) and tms (B). The polymorphism at each marker site was determined by PCR amplification by using a DNA template from the mutant Ler/Col F₁ hybrid (Col ´ mutant) and the controls Ler, Col, and Col/Ler hybrid (Col ´ Ler).