Primer

Designation

Sequence (5′® 3′) or reference

Use

1

ospCflank5’+KpnI

CCTCACTAGGTACCACAGATG

Construction of the ospC mutant

2

ospCflank3’+NdeI

GAGGAGTAATTTCATATGAATC

Construction of the ospC mutant

3

Topo-pCR2.1+BglII-5’

GTACCGAGCTCAGATCTACTAG

Amplification of flaB_P-kan

4

Topo+BglII-3’

GTGATGGAGATCTGCAGAATTC

Amplification of flaB_P-kan

5

P_flgKan.NotI

9

kan probe

6

RC.T_Kan

9

kan probe

7

pc.18

7

Sequencing, screening, ospC probe

8

pc.33

ATAAGTCCTAGAATAAATTA

Sequencing, screening, complementation

9

pc.34

7

Sequencing, screening, complementation

10

pc.38

TAATTTGTGCCTCCTTTTTA

Sequencing, screening

11

pc.41

7

Sequencing, screening, ospC probe