Table 2. Velocity of nuclear migration of Tis21-GFP-positive cells dividing in the ventricular zone, and of their progeny, in slice c
ultures of embryonic day 12.5 telencephalon|
Cell |
Nuclear velocity (µm/h) |
||||
|
Transit |
Migration |
V max average |
V max peak |
||
|
Apical divisions |
Mother |
42.7 ± 10.5 (3) |
55.7 ± 8.3 (3) |
106.5 ± 57.8 (4) |
190 |
|
Daughter A |
24.4 ± 3.0 (3) |
37.3 ± 5.4 (4) |
53.3 ± 7.7 (4) |
63 |
|
|
Daughter B |
16.1 ± 3.7 (3) |
32.1 ± 9.1 (4) |
73.3 ± 30.5 (4) |
103 |
|
|
Basal divisions |
Mother |
10.5 ± 6.9 (3) |
32.9 ± 4.1 (3) |
42.7 ± 9.9 (3) |
54 |
|
Daughter A |
20.2 ± 11.6 (6) |
39.5 ± 14.7 (6) |
55.4 ± 9.8 (6) |
72 |
|
|
Daughter B |
23.1 ± 10.0 (6) |
38.9 ± 16.2 (6) |
60.8 ± 22.7 (6) |
97 |
|
Directions of the migration analyzed were as follows. Apical divisions: mother, apically; daughters, basally. Basal divisions: mother, basally; daughters, basally. Transit, average speed of radial migration from its beginning (mother cell) or from birth (daughter cells) until reaching the final destination or until the end of tracking. Migration, average speed considering only the phases of overt migration. Vmax average, mean value of maximum velocity. Vmax peak, maximum velocity of the fastest cell. For transit, migration, and Vmax average, data are the mean ± SD; the number of cells is indicated in parentheses.