Video 1.
AC-invadopodia turn over dynamically before basement membrane breaching. Ventral view time-lapse shows dynamic F-actin–rich AC-invadopodia (visualized with the F-actin probe cdh-3 > mCherry::moeABD; left) prior to invasion. Images were acquired using a spinning disc confocal microscope (CSU-10 scan head; Yokogawa) mounted on a microscope (AxioImager; Carl Zeiss). Spot tracking analysis was performed in IMARIS 7.4 (Bitplane, Inc.) to quantify invadopodia dynamics (reported in Figs. S1 E and S3 C). A 60-min time-lapse is shown with time points acquired every 15 s. A projection of eight confocal z-sections (step size of 0.5 µm) is shown. The video plays at 10 frames per second. Bar, 5 µm. This video corresponds to the animal shown in Fig. 1 B.