Video 2.
An AC-invadopodium presages and then occupies the site of basement membrane breach. Ventral view time-lapse shows an AC-invadopodium (visualized with cdh-3 > mCherry::moeABD in green) that presages (center, arrowhead) and then occupies the basement membrane breach (right, arrowhead). Images were acquired using a spinning disc confocal microscope (CSU-10 scan head; Yokogawa) mounted on a microscope (AxioImager; Carl Zeiss). A 30-min time-lapse is shown with time points acquired every 15 s. A projection of eight confocal z-sections (step size of 0.5 µm) is shown. Bar, 5 µm. This video corresponds to the animal shown in Fig. 1 E.