Video 5.
UNC-40::GFP enriches at the basement membrane breach. Ventral view time-lapse shows UNC-40::GFP (green) enriching at the site of basement membrane breach (visualized with laminin::mCherry in magenta). Images were acquired using a spinning disc confocal microscope (CSU-10 scan head; Yokogawa) mounted on a microscope (AxioImager; Carl Zeiss). A 60-min time-lapse is shown with time points acquired every 2 min. A projection of eight confocal z-sections (step size of 0.5 µm) is shown. Bar, 5 µm. This video corresponds to the animal shown in Fig. 3 B.