Video 7.
F-actin formation is focused at sites of UNC-40::GFP enrichment. Ventral view time-lapse reveals recurrent F-actin formation (visualized with cdh-3 > mCherry::moeABD in green) at the site-stable UNC-40::GFP (magenta) enrichment. Images were acquired using a spinning disc confocal microscope (CSU-10 scan head; Yokogawa) mounted on a microscope (AxioImager; Carl Zeiss)). A 60-min time-lapse is shown with time points acquired every 15 s. A projection of eight confocal z-sections (step size of 0.5 µm) is shown. Bar, 5 µm. This video corresponds to the animal and time-lapse summation shown in Fig. 5 B.