Video 9.
F-actin formation is not focused at basement membrane breaches in unc-40 mutants. Ventral view time-lapse demonstrates that dynamic F-actin formation (visualized with cdh-3 > mCherry::moeABD in green) is not tightly correlated with the sites of basement membrane breach (visualized with laminin::GFP in magenta) in an unc-40 (e271) mutant animal. Images were acquired using a spinning disc confocal microscope (CSU-10 scan head; Yokogawa) mounted on a microscope (AxioImager; Carl Zeiss). A 60-min time-lapse is shown with time points acquired every 15 s. A projection of eight confocal z-sections (step size of 0.5 µm) is shown. Bar, 5 µm. This video corresponds to the unc-40 (e271) animal and time-lapse summation shown in Fig. 5 C.