Development of a Reverse Transcription-Quantitative PCR System for Detection and Genotyping of Aichi Viruses in Clinical and Environmental Samples

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  Comparison of C_T values for the newly developed RT-qPCR and previously reported assays against cDNA of AiV genotype A and B isolates (Table S1), amplification plots of AiV-universal qPCR assay and genotype-specific duplex qPCR assay with a serial 10-fold dilution of a standard plasmid (Fig. S1), and relationship between serial 10-fold dilutions of extracted AiV-RNA and C_T values obtained from two-step RT-qPCR assay and one-step RT-qPCR assay (Fig. S2).

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