Video 3.
End-link–free closed ends in augmin knockdown cells. Six examples of the closed MT end that has no end-links with an adjacent MT in augmin knockdown cells. Displayed are seven serial slices (1.4-nm-thick slice/frame) and ±10° Y-rotation images (1°/frame). Red crosses in the bottom row indicate tips of closed ends and rotation axes. Images at the top display manual traces of a part of the mother MT wall (green) and the capped end of a daughter MT (magenta) at a sampling plane where MT ends are most clearly seen. The 3DMOD program in the IMOD software package was used for producing tomographic slices and rotating tomograms. ImageJ software was used for making the video from image sequences.