PakB binds to the SH3 domain of Dictyostelium Abp1 and regulates its effects on cell polarity and early development

Supplemental Materials

This article contains the following supporting material:

• Supplemental Materials
• Movie01 - Video S1. Localization of GFP-PakB-1-276 in developed, migrating AX3 cells. GFP-PakB-1-276 localizes to the rear of the migrating cell. Bar, 10 μm.
• Movie02 - Video S2. Localization of GFP-PakB-1-180 in developed, migrating AX3 cells. GFP-PakB-1-180 is enriched at the leading edge of the migrating cells. Bar, 10 μm.
• Movie03 - Video S3. Localization of GFP-PakB-1-180 in growth phase AX3 cells. GFP-PakB-1-180 is enriched in pseudopodia and macropinocytic cups of growth phase AX3 cells. Bar, 10 μm.
• Movie04 - Video S4. Localization of RFP-dAbp1 in developed, migrating AX3 cells. RFP-dAbp1 is enriched at the leading edge of the migrating cells. Bar, 10 μm.
• Movie05 - Video S5. Localization of RFP-dAbp1 in developed, migrating PakB- cells. RFP-dAbp1 exhibits a diffuse distribution in the absence of PakB.