Video 3.
Long-term motion of one locus of chromosome XII in budding yeast. The S. cerevisiae strain (HBT24-1a) bears one labeled locus (position 90 kb), a GFP-tagged nuclear pore complex labeling nuclear envelop (Nup49-GFP), and a red nucleolar marker (mCherry-Nop1). Images were acquired by time-lapse microscopy, using a wide-field microscope (Ti-E/B; Nikon). Frames for GFP were taken every 10 s. Bright-field and mCherry signals were acquired every 100 s. For visualization purposes, red and bright-field images were refreshed every 10 GFP frames. The movie was set to 24 images per second, resulting in an apparent 240-fold speed increase. Note that nuclear size increases with cell cycle progression, and that nuclei move rapidly during mitosis. Images were processed for visualization (see Material and methods).