Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie 1: B. subtilis cells expressing YFP-MreB, Z-stack acquired by SIM (Zeiss), step size 150 nm, going from top to bottom, shown are 6 frames/s.
- Movie02 - Movie 2: B. subtilis cells expressing YFP-MreB, time lapse N-SIM acquisition (exposure time 1.2 s, 3D SIM, 5 s intervals), 6 frames/s
- Movie03 - Movie 3: same field as in movie 2 and same experimental setups, movie acquired 5 min later than movie 2.
- Movie04 - Movie 4: B. subtilis cells expressing YFP-MreB, time lapse N-SIM acquisition (exposure time 1.2 s, 3D SIM, 5 s intervals), 6 frames/s
- Movie05 - Movie 5: B. subtilis cells expressing YFP-MreB, 1 h after arrest of aeration, time lapse epifluorescence acquisition, 5 s intervals, 6 frames/s
- Movie06 - Movie 6: E. coli cells expressing MreB-RFPsw. Time lapse SIM (Zeiss) acquisition (exposure time 1 s, 2D SIM, 5 s intervals), 6 frames/s (note that the size of the movie must be adjusted to properly see the filamentous structures).
- Movie07 - Movie 7: E. coli cells expressing MreB-RFPsw. Time lapse SIM (Zeiss) acquisition (exposure time 1 s, 2D SIM, 5 s intervals), 6 frames/s.
- Movie08 - Movie 8: B. subtilis cells expressing YFP-MreBD158A, time lapse SIM (Zeiss) acquisition (exposure time 0.5 s, 2D SIM, 5 s intervals), 6 frames/s
- Movie09 - Movie 9: B. subtilis cells expressing YFP-MreBD158A, time lapse SIM (Zeiss) acquisition (exposure time 0.5 s, 2D SIM, 5 s intervals), 6 frames/s
- Movie10 - Movie 10: B. subtilis cells expressing YFP-MreB277/305, Z-stack acquired by SIM (Zeiss), step size 150 nm, going from top to bottom, shown are 3 frames/s.
- Movie11 - Movie 11: B. subtilis cells expressing YFP-MreB277/305 for 2 hours, time lapse acquisition by SIM (Zeiss), (exposure time 0.5 s, 2D SIM, 5 s intervals), 6 frames/s
- Movie12 - Movie 12: B. subtilis cells expressing YFP-MreB277/305 for 4 hours, time lapse acquisition by epifluorescence, 5 s intervals, 6 frames/s