Local, cell-nonautonomous feedback regulation of myosin dynamics patterns transitions in cell behavior: a role for tension and geometry?

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie01a - Movie S1a: Time lapse confocal movie to show the transition in the nature of myosin organization (visualized using sqhGFP) between Phase I and Phase II of amnioserosa morphogenesis.
  • Movie01b - Movie S1b: Time lapse confocal movie with simultaneous labeling of membrane (ECadherinGFP) and myoIIDN (driven with AS Gal4).
  • Movie02 - Movie S2: Time lapse confocal movie showing the effect of expression of UAS RhoN19 in a single cell (labeled with actin GFP) on the membrane dynamics (visualized with ECadherin GFP) of the perturbed cell, its NeNe and DiNe.
  • Movie03 - Movie S3: Time lapse confocal movie showing the effect of expression of UAS RhoV14 in a single cell (labeled with actin GFP) on the membrane dynamics (visualized with ECadherin GFP) of the perturbed cell, its NeNe and DiNe.
  • Movie04 - Movie S4: Time lapse confocal movie showing the myosin response (sqhGFP) of NeNe and DiNe of a single mechanically perturbed cell.
  • Movie05 - Movie S5: Time lapse confocal movie showing the effect of expression of UAS RhoN19 in a single cell (yellow dot) on the myosin and membrane dynamics (visualized using sqhGFP and ECadherinGFP respectively) of the perturbed cell, its NeNe and DiNe.
  • Movie06 - Movie S6: Time lapse confocal movie showing the effect of expression of UAS RhoV14 in a single cell (yellow dot)on the myosin and membrane dynamics (visualized using sqhGFP and ECadherinGFP respectively) of the perturbed cell, its NeNe and DiNe.
  • Movie07 - Movie S7: Time lapse confocal movie showing the membrane (ECadhGFP) response of a single mechanically perturbed cell expressing myoIIDN (blue dot).
  • Movie08 - Movie S8: Time lapse confocal movie showing the membrane (ECadhGFP) response of a single mechanically perturbed non-expressing cell surrounded by cells expressing myoIIDN.
  • Movie09 - Movie S9: Time lapse confocal movie showing the membrane (ECadhGFP) and myosin (sqhGFP) response of a naturally delaminating cell (yellow dot) and its NeNe.