Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Supplementary movie 1: Reversibility of different anchor interactions was tested by rapid alternation between the 650 nm and 750 nm lights. Typically 20 cycles were tested. PIF-mCitrine images are shown.
- Movie02 - Supplementary movie 2: Sequestering Clb2 to nucleus (PhyB-HTB2) results in nuclear fission defect. Combined phase and fluorescence time-course (3 min interval) when Clb2 is NOT recruited to the nucleus (750 nm light). Red channel represents the nucleus (PhyB-mCherry-HTB2), and the green channel represents the Spindle Pole Body (Spc42-GFP).
- Movie03 - Supplementary movie 3: Sequestering Clb2 to nucleus (PhyB-HTB2) results in nuclear fission defect. Combined phase and fluorescence time-course (3 min interval) when Clb2 is recruited to the nucleus (with 650 nm light). Red channel represents the nucleus (PhyB-mCherry-HTB2), and the green channel represents the Spindle Pole Body (Spc42-GFP). Most cells fail to split the two nuclei.
- Movie04 - Supplementary movie 4: Sequestering Clb2 to nucleus (PhyB-HTB2) results in nuclear fission defect. Combined phase and fluorescence time-course (3 min interval) when Clb2 is recruited to the nucleus (with 650 nm light). Red channel represents the nucleus (PhyB-mCherry-HTB2), and the green channel represents the Spindle Pole Body (Spc42-GFP). Some cells completely fail to split the nuclei, resulting in two SPBs in the same daughter cell.