Video 5.
Phosphorylations mutants at JIP1-S421 alter KHC activation in vitro. (left to right) KHC-Halo-TMR with wild-type myc-JIP1, myc-JIP1-S421A, and myc-JIP1-S421D. Flow chambers contain KHC-Halo-TMR (red) and microtubules (green). Images were acquired at room temperature at three frames per second for 1 min on a spinning disk TIRF microscope (Ultraview Vox system [PerkinElmer] with Ti body [Nikon]); playback speed is 10× real time (30 frames per second)