Video 2.
brpΔ170 T-bar structure. Electron microscope tomography reconstruction of brpΔ170 larval NMJ terminals. Sections of second instar larvae (250 nm thick) were prepared by HPF/FS. Tilt series were acquired on a microscope (Tecnai Spirit) at a nominal magnification of 30,000 at 3–5-µm defocus using Xplore3D.