Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Kennedy et al. (July 10, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.151240898

Supplemental Figure 5 Fig. 5.
Deacetylase components required for repression by Ume6p. (A) The reporter used in this assay has 10 Gal4 binding sites and the URS element from the SPO13 promoter that is a natural binding site for the Ume6p. (B) Components of deacetylase machinery required for repression by Ume6p. Strains do not have Gal4DB fusions and do not display dramatic differences in growth rate on media lacking 5-FOA. Strains with reduced URA3 expression are able to grow in the presence of 2 g/l 5-FOA. The plate was photographed after 3 days.