Supplementary material for Fuchs et al. (July 3, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.121433898
Table 1. PCR primers used for amplification of the various members of the Rho family
GTPase | Accession | up-Primer (EcoRI) | do-Primer (XhoI) |
RhoA | L25080 | 5'-GCA GAA TTC GGC ACC ATG GCT GCC ATC CGG AAG AAA CTG GTG-3' | (NotI) 5'-GCA CGC CGG CGT CAC AAG ACA AGG CAA CCA GAT TTT TTC T-3' |
RhoB | NM_004040 | 5'-GCA GAA TTC GGC ACC ATG GCG GCC ATC CGC AAG AAG CTG-3' | 5'-GCA CTC GAG GTA GTC GTA GGC TTG GAT G-3' |
RhoC | NM_005167 | 5'-GCA GAA TTC GGC ACC ATG GCT GCA ATC CGA AAG AAG CTG GTG-3' | 5'-GCA CTC GAG TCA GAG AAT GGG ACA GCC CCT CCG ACG-3' |
RhoG | NM_001665 | 5'-GCA GAA TTC GGC ACC ATG CAG AGC ATC AAG TGC GTG GTG GTG-3' | 5'-GCA CTC GAG TCA CAA GAG GAT GCA GGA CCG CCC ACG-3' |
cdc42 | NM_001791 | 5'-GCA GAA TTC GGC ACC ATG CAG ACA ATT AAG TGT GTT GTT GTG G-3' | 5'-GCA CTC GAG TCA TAG CAG CAC ACA CCT GCG GCT CTT C-3' |
TTF | NM_004310 | 5'-GCA GAA TTC GGC ACC ATG CTG GAG TTC CAT CAA GTG CGT GTT G-3' | 5'-GCA CTC GAG TTA GAA ATC TTG CAC TCA TTG ATG GAG-3' |
Rac1 | NM_006908 | 5'-GCA GAA TTC GGC ACC ATG CAG GCC ATC AAG TGT GTG GTG GTG-3' | 5'-GCA CTC GAG TTA CAA CAG CAG GCA TTT TCT CTT CCT C-3' |
Rac2 | NM_002872 | 5'-GCA GAA TTC GGC ACC ATG CAG GCC ATC AAG TGT GTG GTG-3' | 5'-GCA CTC GAG CTA GAG GAG GCT GCA GGC GCG-3' |