Live-Cell Imaging Tool Optimization To Study Gene Expression Levels and Dynamics in Single Cells of Bacillus cereus

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Slide preparation method (in detail); primers used in this study (Table S1); fluorescence signals of various FP variants in B. cereus ATCC 14579 (Fig. S1).

  PDF, 313K

• Supplemental file 2 -

  Time-lapse movie of growth and development of B. cereus ATCC 14579 (Movie S1).

  AVI, 6.8M

• Supplemental file 3 -

  GFP expression in single cells of B. cereus ATCC 14579, expressed from a chromosomally integrated single-copy cotD promoter (Movie S2).

  AVI, 3.7M

• Supplemental file 4 -

  GFP expression in single cells of B. cereus ATCC 14579, expressed from a cotD promoter on a multicopy vector (Movie S3).

  AVI, 3.7M