In Vivo Studies Suggest that Induction of VanS-Dependent Vancomycin Resistance Requires Binding of the Drug to d-Ala-d-Ala Termini in the Peptidoglycan Cell Wall

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Table S1, bacterial strains, plasmids, and primers used in this study. Figure S1, integrative reporter plasmid pMK2 carrying the neo neomycin/kanamycin resistance gene expressed under the control of the vancomycin-inducible vanJ promoter. Figure S2, D-Lac supplementation alters the ratio of D-Ala-D-Ala:D-Ala-D-Lac-containing PG precursors in strains expressing the VanA ligase and increases the concentration of vancomycin required for survival of the vancomycin-dependent ΔddlA mutant strain.

  PDF, 2.3M