Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie S1. Dynamics of reggie-tubules in HeLa cells. HeLa cells were transfected with reggie- 1-EGFP and recorded using a Colibri imaging system. Tubular structures are highly dynamic and can be seen to derive from the perinuclear recycling compartment and move towards the PM as well as from the PM to the recycling compartment. Vesicles can be seen to fuse with the PM and travel back to the recycling compartment.
- Movie02 - Movie S2. Dynamics of reggie-tubules in A431 cells. A431 cells were transfected with reggie- 1-EGFP and recorded as in Movie S1. Reggie-tubules are highly dynamic and move to and from the PM. Vesicles are observed traveling through the cell to the PM and back.
- Movie03 - Movie S3. Trafficking of E-cadherin in reggie-tubules in A431 cells. A431 cells were transfected with reggie-1-mRFP and E-cadherin-EGFP and recorded as in Movie S1 at 60 min after Ca2+ repletion (Ca2+ switch experiment). Both proteins are observed at the perinuclear recycling compartment and trafficking in tubules and vesicles.