Video 2.
Migration of NSC23766-, Y-27632–, or blebbistatin-treated CHO-α4WT cells through 50- and 6-µm channels. After 1-h incubation of CHO-α4WT cells with the indicated inhibitor or vehicle control at 37°C, 10% FBS was added to the topmost (chemotactic) inlet port to induce migration. Images were acquired by time-lapse microscopy using an inverted microscope (Eclipse Ti). Frames were taken every 20 min for 8 h. The video accompanies Fig. 3 A. Time shows hours and minutes. Bleb, blebbistatin; NSC, NSC23766.